Effects of stress-induced acute depression and antidepressant drugs on CA3 region of hippocampus of albino rats

Abstract

Depressive disorders are amongst the most common life threatening disorders and despite of extensive research work its etiology and mode of action of antidepressant drugs remains elusive. The present study was conducted on 15 adult albino rats (200-250gm) divided into control and experimental groups. First group was control, second group received acute depression (4 weeks) by immobilization method using rat immobilizer and third group received standard 4 week treatment (using Flouxetine 1mg/kg body wt. orally) following acute depression. At the end of the experiment animals were sacrificed and perfused with 10% formaldehyde. Brains were dissected and tissue blocks were processed for paraffin embedding. Observations were made on 10 micron thick H & E stained sections. Estimation of neuronal density of CA3 regions was performed using Motic images plus 2.0 Software. Neuronal density was markedly reduced (100.3 cells/cubic mm) after acute depression, as compared to control (144.5 cells/cubic mm) and after standard treatment it improved to 121.1 cells /cubic mm. These results suggested that effect of short-term stress-induced depression on hippocampus is partly reversed by the pharmacological intervention of known antidepressants and warrants longer term study.

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